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Fig. 4 | Cell & Bioscience

Fig. 4

From: Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri‐mir‐375 to miR-375

Fig. 4

ShRNA-Mettl14 inhibited H2O2-induced apoptosis in SCI cell model. C8-DA1 and C8-B4 cells induced by H2O2 were used to establish the cell model of SCI in vitro. Mettl14 expression was detected by RT-qPCR (a). The content of m6A in the total RNA of the spinal cord was measured by EpiQuik m6A RNA methylation quantification kit (b). Then flow cytometry (c) and Hoechst staining (d) were used to evaluate the apoptosis. Each experiment was repeated three times independently. The data are expressed by mean ± standard deviation. The data in panels A/B/C were analysed by one-way ANOVA, followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01

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