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Fig. 1 | Cell & Bioscience

Fig. 1

From: Mettl14-mediated m6A modification modulates neuron apoptosis during the repair of spinal cord injury by regulating the transformation from pri‐mir‐375 to miR-375

Fig. 1

Mettl14 and m6A were significantly upregulated after SCI. After the SCI model was established, the motor function of rats in each group was assessed by BBB motor function score (a) and bevel test score (b) on the 0, 5, 10, 15, 20, 25 and 30 days. The SCI at 2-cm transverse section of the spinal cord centred on the injured site was assessed by HE staining (c) on the 30th day; the neurons at the point of arrow were obviously lost; and the m6A content in total RNA of rats at the 5th day, 10th day and 30th day after SCI was assessed by EpiQuik m6A RNA Methylation quantification kit (d). The expression of Mettl14 at the 5th day, 10th day and 30th day after SCI was detected by RT-qPCR (e) and Western blot (f); and the fluorescence intensity of Mettl14 in a 2-cm transverse section of the spinal cord centred on the injured site on the 30th day was detected using immunofluorescence (g), and the arrow indicates Mettl14 positive expression. N = 8. Each experiment was repeated three times independently, and the data are expressed as mean ± standard deviation. The data in panels a, b were analysed by two-way ANOVA, and data in panels df were analysed by one-way ANOVA, followed by Tukey’s multiple comparisons test. Compared with the sham group, *p < 0.05, **p < 0.01

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