Fig. 3

Selonsertib reverses JNK-mediated DRP1 mitochondrial translocation and abnormal mitochondrial dynamics. a, b Western blot analysis and grey value assay of p-DRP1 and DRP1 levels in murine whole liver samples (a) and the mitochondrial fraction of liver samples (b) at initial (0 h) and 0.5 h, 1 h, 2 h, and 4 h after LPS/GalN injection. c, d Western blot analysis and grey value assay of p-DRP1 and DRP1 levels in murine whole liver samples (c) and the mitochondrial fraction of liver samples (d) from different groups. e Western blot analysis and grey value assay of the expression levels of mitochondrial dynamics-related proteins in murine liver samples. f–h Anisomycin was used to further confirm the role of JNK in mediating the effects of selonsertib on DRP1 activation and mitochondrial dynamics. Western blot analysis and grey value assay of DRP1 activation and mitochondrial translocation in murine whole liver samples (f) and the mitochondrial fraction of liver samples (g) and the expression levels of mitochondrial dynamics-related proteins in murine liver samples (h). Two from 6 samples were shown in the blots. Data are presented as the mean ± SD (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; NC normal control, SEL selonsertib (30 mg/kg), AN anisomycin (20 mg/kg)