Fig. 4

MSCs derived from hESCs through a heterogeneous intermediate stage. a Dynamic morphology of derived hPSC-MSCs. Scale bar = 20 μm. b Representative immunofluorescence images of β-tubulin in hPSC-MSCs. Scale bar = 50 μm. c, d qRT-PCR (c) and western-blotting (d) analyses of pluripotency-associated gene expression in hESCs and derived cells. e–f qRT-PCR (e) and western-blotting (f) analyses of germ layer-associated gene expression in hESCs and derived cells. g, h qRT-PCR (g) and western-blotting (h) analyses of MSC-associated gene expression in hESCs and derived cells. All data were shown as mean ± SEM (N = 3). *P < 0.05, **P < 0.01; NS, not significant