Fig. 1

Identification of new markers in endothelial cells after CCM3 knockdown. a mRNA changes of electron transport chain and OXPHOS genes from RNA-seq in HUVECs after siNC and siCCM3 treatment by qRT-PCR. b-c protein changes of OXPHOS in HUVECs by Western Blotting (b), and the quantification of each band (c), β-actin was blotted as internal control. mRNA changes of cell–cell adhesion (d) and angiogenesis (e) pathway genes from RNA-seq results in HUVECs, the significantly changed genes were also examined by Western Blotting (f) and quantified (g). qRT-PCR results were expressed as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001