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Fig. 4 | Cell & Bioscience

Fig. 4

From: KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity

Fig. 4

KLHL18 interacts with and promotes the ubiquitination and degradation of PI3Kp85α. a Co-IP of KLHL18 with PI3KP85α in NCI-A549 and NCI-H1299 cells. b Immunofluorescence assay; KLHL18 co-localized with PI3KP85α in NCI-A549 and NCI-H1299 cells. c KLHL18 protein co-immunoprecipitated with CUL3 protein in HEK-293 T cells. d, e KLHL18 inhibited the expression of PI3Kp85α via the Rbx1–CUL3–KLHL18 complex in d NCI-A549 and (E) NCI-H1299 cell lines. The lower graph corresponds to the gray value statistical graph, *P < 0.05, **P < 0.01. f Silencing KLHL18 protein inhibited the ubiquitination of PI3Kp85α protein. g An increase in KLHL18 protein expression levels promoted the ubiquitination-mediated degradation of PI3Kp85α protein

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Cell & Bioscience

ISSN: 2045-3701

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