Table 1 Stem-loop RT and RT-quantitative polymerase chain reaction primer sequences
From: Lrp4 in hippocampal astrocytes serves as a negative feedback factor in seizures
Gene | Sequence (5′ → 3′) |
|---|---|
U6 (F) | CTCGCTTCGGCAGCACA |
U6 (R) | AACGCTTCACGAATTTGCGT |
mmu-miR-125a-5p (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAGGT |
mmu-miR-351-5p (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACCAGGCTCA |
mmu-miR-6367 (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCCTGAAC |
mmu-miR-125b-5p (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAAGT |
mmu-miR-6394 (RT) | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAGACCTGG |
mmu-miR-125a-5p (F) | CGCGTTCCCTGAGACCCTTTA |
mmu-miR-351-5p (F) | CGTTCCCTGAGGAGCCCTT |
mmu-miR-6367 (F) | AGCGTTCCCTGAGACCCTG |
mmu-miR-125b-5p (F) | CGCGTTCCCTGAGACCCTA |
mmu-miR-6394 (F) | AGCGTTCCCTGAGTGGGG |
General mmu-miR (R) | ATCCAGTGCAGGGTCCGAGG |