Skip to main content
Fig. 4 | Cell & Bioscience

Fig. 4

From: Lrp4 in hippocampal astrocytes serves as a negative feedback factor in seizures

Fig. 4

miR-351-5p inhibitor attenuated the reduction of Lrp4 expression by glutamate treating in cultured hippocampal astrocytes. a Relative level of mmu-miR-351-5p in hippocampal astrocytes which were transfected respectively by miR-351-5p mimics, miR-351-5p m NC (mimics negative control), miR-351-5p inhibitor, miR-351-5p I NC (inhibitors negative control). miR-351-5p mimics enhanced the miR-351-5p level and miR-351-5p inhibitors weakened the miR-351-5p level. b Relative level of Lrp4 mRNA in primary cultured hippocampal astrocytes which were transfected with miR-351-5p mimics at different concentration (0 nM, 25 nM, 50 nM, 100 nM). Lrp4 mRNA level decreased at the test concentration of the mimics (25 nM, 50 nM, 100 nM). c Lrp4 protein level in cultured astrocytes after transfecting miR-351-5p mimics by Western blotting. Action as an internal control. d Quantification of Lrp4 protein level from panel C, miR-351-5p mimics cut down Lrp4 protein expression. e mRNA level of Lrp4 in astrocytes after glutamate treatment only or glutamate treatment plus miR-351-5p inhibitors transfection. mRNA level of Lrp4 in astrocytes decreased about 70% by glutamate treating, and decreased about 25% by glutamate plus miR-351-5p inhibitors treating. f Lrp4 protein level in cultured astrocytes after glutamate treatment only or glutamate treatment plus miR-351-5p inhibitors transfection, Actin as an internal control. g Quantification of Lrp4 protein level from f, Lrp4 protein in astrocytes decreased about 70% by glutamate treating and decreased about 25% by glutamate plus miR-351-5p inhibitors treating. For each experiment, three separate experiments were performed in duplicate (**p < 0.01)

Back to article page