Fig. 3

Seizures increased miR-351-5p in vivo and glutamate increased miR-351-5p in vitro through activating NMDA receptor. a Relative level of microRNA in hippocampus, injecting pilocarpine into mice. 4 h after injection, the relative level of mmu-miR-351-5p in hippocampus was increased. Two separate experiments were performed in triplicate (**p < 0.01). b Time course of the mmu-miR-351-5p relative level in hippocampus after injection, mmu-miR-351-5p increased at each time point (2 h, 4 h, 12 h) after injection. c Time course of the mmu-miR-351-5p relative level in hippocampus after glutamate treatment. mmu-miR-351-5p increased at each time point (2 h, 4 h, 12 h) after treating. d Relative level of mmu-miR-351-5p expression in hippocampal astrocytes which were treated with glutamate (0.5 mM) only or glutamate (0.5 mM) plus AP5 (50 mM). After 4 h treating, the elevation of mmu-miR-351-5p induced by glutamate plus AP5 treating is significantly lower than that induced by glutamate treating. For each experiment, three separate experiments were performed in duplicate (**p < 0.01)