Fig. 2

Glutamate decreased Lrp4 expression through activating NMDA receptor in cultured hippocampal astrocytes. a Relative mRNA of Lrp4 in cultured hippocampal astrocytes after glutamate treatment, which were collected at different time point (0 h, 2 h, 4 h, 12 h) after treatment. Lrp4 mRNA decreased after glutamate treatment (2 h, 4 h, 12 h). b Protein level of Lrp4 in cultured hippocampal astrocytes after glutamate treatment, which were collected at different time point (0 h, 2 h, 4 h, 12 h) after treatment, Actin as an internal control. Lrp4 protein decreased after glutamate treatment (4 h, 12 h). c Quantification of Lrp4 protein level in b. d Relative mRNA level of Lrp4 in primary cultured astrocytes which were treated with glutamate (0.5 mM) only or glutamate (0.5 mM) plus AP5 (50 mM). After 12 h, Lrp4 mRNA decreased about 75% after glutamate treatment, but decreased about 20% after glutamate plus AP5 treatment. e Lrp4 protein level in cultured astrocytes which were treated with glutamate (0.5 mM) only or glutamate (0.5 mM) plus AP5 (50 mM). Actin as an internal control in Western blotting. f Quantification of Lrp4 protein level in e, Lrp4 protein decreased about 70% after glutamate treatment, but decreased about 25% after glutamate plus AP5 treatment. For each experiment, three separate experiments were performed in duplicate (**p < 0.01)