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Fig. 6 | Cell & Bioscience

Fig. 6

From: CXCR7: a β-arrestin-biased receptor that potentiates cell migration and recruits β-arrestin2 exclusively through Gβγ subunits and GRK2

Fig. 6

CXCR7 and CXCR4 recruitment of β-arrestin2 is GRK-dependent. a, b HEK293 cells expressing NanoBiT constructs for each receptor and β-arrestin2 were pre-treated with different doses of the GRK2-specific inhibitor Cmpd101, and then stimulated with their cognate chemokines. Luciferase activities were then measured. c, d Bar graphs show maximum luciferase activities in each group pre-treated with different doses of Cmpd101. Values are presented as the mean ± SD. **p < 0.01, **p < 0.001. e HEK293 cells expressing exogenous CXCR4 were pre-treated with Cmpd101, and then stimulated with SDF-1α. Cell lysates were used for western blotting with anti-pERK1/2 or ERK antibodies. Results are the average of three independent experiments

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