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Fig. 1 | Cell & Bioscience

Fig. 1

From: CXCR7: a β-arrestin-biased receptor that potentiates cell migration and recruits β-arrestin2 exclusively through Gβγ subunits and GRK2

Fig. 1

SDF-1α induced β-arrestin2 recruitment to CXCR4 and CXCR7. a Schematic representation of the structural complementation assays for the interaction of β-arrestin2 with chemokine receptors b HEK293 cells, transiently transfected with constructs for receptor-LgBiT and SmBiT-β-arrestin2, were treated with the indicated chemokines (I-TAC/SDF-1α) or not (Veh.), and then assessed for real-time luminescence. c CXCR4 and CXCR7 mediate intracellular clustering of β-arrestin2 by SDF-1α. Cells transfected with β-arrestin2-GFP and chemokine receptor constructs were treated with SDF-1α for 30 min. GFP fluorescence was then assessed using confocal microscopy. d Translocation of CXCR4 and CXCR7 to the cytosol by SDF-1α. Cells expressing CXCR4-GFP or CXCR7-GFP were treated with SDF-1α and fixed. Confocal images show the subcellular localization of GFP signals (left panels). Cells expressing HA-tagged receptors were labeled with anti-HA antibodies and treated with SDF-1α for 30 min (NT: no treatment). Fixed cells were permeabilized and stained with FITC-conjugated goat anti-mouse IgG. The images show the subcellular localization of the HA-tagged receptors (right panel). e Cell surface expression of the receptors. Transfected cells with constructs for HiBiT-tagged receptors were incubated with extracellular HiBiT detection reagent, and luminescence was measured. The results are an average of three independent experiments. Values are presented as the mean ± SD

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