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Fig. 3 | Cell & Bioscience

Fig. 3

From: Enhancing the antibacterial activities of sow milk via site-specific knock-in of a lactoferrin gene in pigs using CRISPR/Cas9 technology

Fig. 3

Detection of the antibacterial activity of milk from pLF-KI pigs in vitro. a Raw components of genetically modified colostrum compared with wild-type colostrum. b Detection of the expression of LF in colostrum stage and milk stage in pLF-KI pigs by qRT-PCR assay. c Western blot analysis for the expression and accumulation of LF protein between pLF-KI pigs and wild-type pigs. KD, Kilodalton. d Bacterial plate assay for Escherichia coli (E. coli) survival in response to colostrum treatment. Depletion zones developed on a lawn of E. coli 12 h after sample application: (1) 10 μL milk from genetically modified pig, (2) 10 μL milk from wild-type pig, (3) 10 μL sterile water and (4) 10 μL antibiotic. e Plate dilution colony counting assay to test the antibacterial activity of colostrum. pLF-KI, porcine lactoferrin knock-in; WT, wild-type; Anti, Antibiotic; CFU, colony-forming unit; Blank: sterile water; the cycles in orange indicate the transparent zones. **P < 0.01, ***P < 0.001, ****P < 0.0001; P values were determined by two-sided Student’s t test

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