Fig. 4
Pla2g10 promoter has functional PREs in uterine epithelial cells. a Luciferase assays for a proximal region of Pla2g10 promoter in Ishikawa cells where control (Vec), PRA, or PRB vectors were co-transfected with pGL4.10/Pla2g10 luciferase vector under 1 µM P4. Vec: empty vector. b A schematic cartoon to show Pla2g10 promoter that contains putative PREs that were mutated by site-directed mutagenesis. Box, PRE sequence. Red letter, mutated sequence. c Luciferase assays for Pla2g10 promoter constructs with a mutation (mt) on each PRE in Ishikawa cells under the same conditions as a. The normalized luciferase activities are shown by ratio to renilla activity. Data are presented as the mean ± SD of three independent experiments. *p < 0.05