Fig. 1
Pla2g10 expression is positively regulated by P4 in the uterus. a A volcano plot to compare expression profiles of the mid-luteal phase endometria from the healthy women (CON, n = 6) vs patients with RIF (n = 9). PLA2G10 is visualized as one of downregulated genes in the endometria of patients with RIF. b Real-time RT-PCR analysis for relative mRNA levels of PLA2G10 between endometria of CON and patients with RIF in mid-luteal phase. c Immunofluorescence of PLA2G10 24 h after P4 treatment in uteri of OVX mice. The bottom panels show higher magnification images of the boxed area. Green and red colors indicate the presence of PLA2G10 and nuclei, respectively. LE, luminal epithelium; GE, glandular epithelium; S, stroma. Scale bar: 50 µm. d–f RT-PCR and real-time RT-PCR results to analyze relative levels of Pla2g10 mRNA by d P4 (2 mg/mouse), e E2 (200 ng/mouse), and f E2 + P4 treatment in uteri of OVX mice at different time points (n = 4–5 mice for each time point). Expression levels of Areg and LF mRNAs were also evaluated to validate appropriate P4 and E2 hormone responses in OVX mice used in this experiment, respectively. *p < 0.05