Fig. 6From: Mesenchymal stem cells desensitize castration-resistant prostate cancer to docetaxel chemotherapy via inducing TGF-β1-mediated cell autophagyDocetaxel increases TGF-β1 secretion of MSCs when co-cultured with CRPC cells a ELISA assay was performed to examine the concentration of TGF-β1 in coculture medium of MSCs and PCa cells after DTX administration. b The amount of TGF-β1 in conditioned medium was detected by ELISA. c TGF-β1 mRNA in MSCs was assayed by real-time PCR. d The expression of TGF-β1 in protein level was examined by western blot. e Immunohistochemistry against TGF-β1 in tumour tissues was observed by microscope (original magnification ×200). **P < 0.01Back to article page