Fig. 4

Chromatin immunoprecipitation assays. a Schematic illustration showing primer positions of dnmt3aa gene in the ChIP assay. P1 region and P2 region were target region and control region, respectively. b Chromatin immunoprecipitation in vivo. Foxa1 can bind to the dnmt3aa promoter in testis. P2 region was used as a control. Sonicated chromatin from testis were immunoprecipitated with anti-Foxa1, beads only, and preimmune IgG (control). A 150 bp fragment corresponding to the −81 to +69 region was amplified using the immunoprecipitated DNA as a template. PCR amplification of Exon 4 of dnmt3aa was used as a negative control