Fig. 3

Mutation analysis of Foxa1 binding sites in the dnmt3aa promoter. a Sequence logo of Foxa1 binding site predicted by JASPAR database (jaspar.genereg.net) with core sequence [T(A/G)TT(T/G)]. b Schematic showing the DNA sequences of dnmt3aa mutants and wild type. Mut a, b and a/b indicated mutant site a (red), b (blue), and a/b double mutant respectively. c Point mutation analysis of the promoter using luciferase assays in 293T (top panel) and COS7 (bottom panel) cells. Both mutations a and b can lead to obviously decrease of luciferase activity, and the decrease was most apparent in double mutant. The mean ± SEM were from 3 independent experiments, *P < 0.05; **P < 0.01. d Overexpression of foxa1 up-regulated dnmt3aa promoter activities in 293T (left panel) and COS7 (right panel) cells. The mean ± SEM were from 3 independent experiments, *P < 0.05; **P < 0.01