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Fig. 2 | Cell & Bioscience

Fig. 2

From: DNA methylation modification is associated with gonadal differentiation in Monopterus albus

Fig. 2

Activity analysis of the dnmt3aa promoter. a Schematic showing the position of Foxa1 binding sites and CpG sites. Two Foxa1 binding sites, a from − 13 to − 20, and b from − 22 to − 29; CpG sites were represented by short bars. b Luciferase assay showing the activities of a series of deleted constructs in 293T (top panel) and COS7 (bottom panel) cells. Left part of each panel showed each deleted mutant linked with the luciferase gene in the pGL3-basic vector. Right part indicated the relative activities of these deleted constructs. The mean ± SEM were from 3 independent experiments, *P < 0.05; **P < 0.01. c Expression pattern of dnmt3aa and foxa1 among gonads analyzed by semi-quantitative RT-PCR. hprt (hypoxanthine phosphoribosyltransferase) was employed as an internal control

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