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Fig. 6 | Cell & Bioscience

Fig. 6

From: Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury

Fig. 6

MAPK4 deficiency reduces the expression of inflammatory cytokines and transduction of related signaling pathway in macrophages. a Peritoneal macrophages were collected and FCM was used to verify the purity. b The mRNA levels of MAPK4 were detected by Real-time PCR assay in WT macrophages and LPS-treated WT macrophages (n = 3). c, d The protein levels of MAPK4 in WT macrophages and LPS-treated WT macrophages were detected by immunofluorescence and calculated (n = 3). e The mRNA levels of IL-1β and TNF-α in LPS-treated WT and MAPK4−/− macrophages were detected by Real-time PCR assay (n = 3). fg The expression of p-p38 MAPK was detected by FCM and calculated. Scale bar = 25 μm. Data were presented as the mean ± SEM. *p < 0.05, **p < 0.01

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