Fig. 4

MAPK4 is up-regulated in the lung tissues of murine ALI. a WT C57BL/6 mice (n = 5–6) were administered with i.p. 10 mg/kg LPS or PBS. Lung tissues were obtained at different time points. b The mRNA levels of MAPK4 in lung tissues were detected by Real-time PCR assay. c The protein levels of MAPK4 in lung tissues were analyzed by Western blot and calculated. d–g The expressions of MAPK4 in F4/80⁺ cells in lung tissues were analyzed by immunofluorescence and calculated, respectively. Arrows indicate F4/80⁺ cells. Scale bar = 50 μm. Data were presented as the mean ± SEM. **p < 0.01