Fig. 3

MAPK4 deficiency alters the transduction of related signaling pathways in ALI. WT C57BL/6 mice (n = 5–6) and MAPK4^−/− mice (n = 5–6) mice were administered with i.p. 10 mg/kg LPS, respectively. After 24 h, lung tissues were obtained. a, b The expressions of MAPK4, MK5 and p-MK5 were detected by Western blot and calculated. c, d The expressions of p-MK5 were detected by immunohistochemistry and calculated. e, f The expressions of ERK, p-ERK, AKT, p-AKT, p-NF-κB, p38 MAPK, p-p38 MAPK, JNK and p-JNK were detected by Western blot and calculated. Scale bar = 25 μm. Data were presented as the mean ± SEM. *p < 0.05, **p < 0.01