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Fig. 6 | Cell & Bioscience

Fig. 6

From: Inducible expression of heat shock protein 20 protects airway epithelial cells against oxidative injury involving the Nrf2-NQO-1 pathway

Fig. 6

The influence of HSP20 and its phosphorylation on the Nrf2-NQO-1 expression in BEAS-2B cells. BEAS-2B cells were transfected with pEX-3, pEX-3-HSP20, and pEX-3-HSP20(Ala) and then stimulated with H2O2 (10 μM) for 1 h. a The mRNA expressions of NQO-1 measured with RT-qPCR. b The protein expression of NQO-1 and GADPH (control) in whole cell lysis and c Nrf2 and Lamina A/C (control) in nuclear extractions were measured with Western blot, respectively. Data are expressed as mean ± SD on three independent experiments

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