Fig. 6

The influence of HSP20 and its phosphorylation on the Nrf2-NQO-1 expression in BEAS-2B cells. BEAS-2B cells were transfected with pEX-3, pEX-3-HSP20, and pEX-3-HSP20(Ala) and then stimulated with H2O2 (10 μM) for 1 h. a The mRNA expressions of NQO-1 measured with RT-qPCR. b The protein expression of NQO-1 and GADPH (control) in whole cell lysis and c Nrf2 and Lamina A/C (control) in nuclear extractions were measured with Western blot, respectively. Data are expressed as mean ± SD on three independent experiments