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Fig. 5 | Cell & Bioscience

Fig. 5

From: Inducible expression of heat shock protein 20 protects airway epithelial cells against oxidative injury involving the Nrf2-NQO-1 pathway

Fig. 5

The in vitro protective effect of HSP20 on BEAS-2B cells. BEAS-2B cells were transfected with pEX-3, pEX-3-HSP20, and pEX-3-HSP20(Ala) and then stimulated with H2O2 (10 μM) for 1 h. a The expression of HSP20 mRNA measured with RT-qPCR. b The phosphorylated and total HSP20 protein measured with Western blot. c The intracellular content of reactive oxidative species (ROS) measured with DCFH-DA-based method. d Cell apoptosis measured with FACS counting on the Annexin V-FITC and PI stained cells and the early cell apoptosis (e) and cell necrosis (including late cell apoptosis) (f) were calculated. Data are expressed as mean ± SD

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Cell & Bioscience

ISSN: 2045-3701

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