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Fig. 4 | Cell & Bioscience

Fig. 4

From: Beta-catenin inhibits TR4-mediated lipid accumulation in 3T3-L1 adipocytes via induction of Slug

Fig. 4

Slug downregulates TR4 transcriptional activity via direct interaction with TR4. a, c. Interaction between TR4 and Slug was analyzed using GST pull-down assay. Equal amounts of in vitro translated 35S-labled Slug or 35S-labled TR4 were incubated with purified GST fusion proteins bound to glutathione-Sepharose beads, as indicated. b A mammalian two-hybrid assay was performed in HEK293T cells by co-transfection of the pG5-Luc plasmids with different Gal4-Slug constructs and VP16-TR4, as indicated. The different Gal4-Slug constructs used for this assay are illustrated schematically on the upper panel. d A reporter plasmid was co-transfected into HEK293T cells along with TR4 expression plasmids and different Slug fragments, as indicated. (***p < 0.001)

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