Fig. 2
From: Beta-catenin inhibits TR4-mediated lipid accumulation in 3T3-L1 adipocytes via induction of Slug
Slug is required for β-catenin-mediated suppression of TR4 activity. a RT-qPCR analyses of gene expression in day 5 3T3-L1-β-catenin or 3T3-L1-pCI adipocytes. b and d. Luciferase assays in the HEK293T (FATP1-DR1-Luc and PC-DR1-Luc) and NIH-3T3 (FATP1pro-Luc and PCpro-Luc) cells after transfection of reporter plasmids along with TR4, Snail, Slug, SlugmiR, or β-catenin expression plasmids, as indicated. c Immunoblots of Slug and β-actin during 3T3-L1 adipogenesis. e RT-qPCR analyses of Fatp1 and Pc mRNA levels in 3T3-L1-C and 3T3-L1-TR4 adipocytes at day 8 after 48 h transfection with SlugmiR or β-catenin expression plasmids as indicated. (**p < 0.005, and ***p < 0.001)