Fig. 1
From: Beta-catenin inhibits TR4-mediated lipid accumulation in 3T3-L1 adipocytes via induction of Slug
β-catenin inhibits TR4 target gene expression by repressingTR4 transcriptional activity. a Immunoblots of β-catenin, TR4, aP2, and β-actin during 3T3-L1 adipogenesis. b Effect of β-catenin on the expression of TR4 target genes. Protein levels of β-catenin, TR4, PC, FATP1, CD36, and β-actin were analyzed by immunoblotting in β-catenin-overexpressing (3T3-L1-β-catenin) or control 3T3-L1 (3T3-L1-pCI) adipocytes at day 5. c Luciferase assays in HEK293T or NIH-3T3 cells after transfection of reporter plasmids along with TR4 and β-catenin expression plasmids, as indicated. (*p < 0.01, **p < 0.005, and ***p < 0.001 vs. control) d Interaction between TR4 and β-catenin was analyzed with a GST pull-down assay. The different GST-TR4 constructs used were described previously [24] and are illustrated schematically on the upper panel. Input: 20% of the labeled protein used in the GST pull-down assay