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Fig. 1 | Cell & Bioscience

Fig. 1

From: Immunoprecipitation and mass spectrometry define TET1 interactome during oligodendrocyte differentiation

Fig. 1

Immunoprecipitation and mass spectrometry revealed endogenous TET1 interactors in oligodendrocytes. a Immunostaining with lineage specifc oligodendrocyte makers indicated the purity of oligodendrocyte cultures. Antibody against PDGFRα labeled OPCs and against MBP labeled mature OLs. Scale bar, 50 μm. b Quantification the percentage of PDGFRα+ and MBP+ cells in OPC and OL stage, respectively. c Westernblot assay identified TET1 in TET1-IP products in both OPC and OL groups. Red arrows indicate the predicted bands for endogenous TET1. IP input from both groups was used as positive control for TET1. β-actin was used as loading control and negative control. TET1 did not appear in IgG-IP products. d Coomassie blue staining of SDS-PAGE for TET1-IP products from OPC and OL samples. e Analysis workflow for the filtering of MS data. Specificity, reliablity and contamination filteration result a final dataset of 1211 proteins in OPC and OL samples

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