Fig. 4

Cofilin activity mediates NRP2-driven HUVEC migration and actin organization. A HUVEC-vector and HUVEC-NRP2 cells were transfected with scramble or cofilin siRNA. a Cells were lysed and subjected to Western blot analysis with cofilin antibodies. b The migratory properties of the cells were analysed by the wound-healing assay (***P ≤ 0.001 by Student’s t test). The data are presented as averages from three independent experiments. B The fluorescent signals of F-actin (green) and nuclei (blue) are shown (× 1000). C HUVEC-vector and HUVEC-NRP2 cells were transfected with cofilin S3A or S3E before they were lysed and subjected to Western blot analysis with the indicated antibodies. D HUVEC-NRP2 cells were transfected with cofilin S3A (a) or cofilin S3E (b). The migratory properties of the cells were analysed by the wound-healing assay. The data are summarized from three independent experiments