Fig. 1
From: Rap1b but not Rap1a in the forebrain is required for learned fear
Generation of forebrain-specific Rap1a or Rap1b knockout mice. a Genomic DNA PCR genotyping of Rap1af/f, Rap1bf/f, Rap1a cKO and Rap1b cKO mice. Arrows indicate bands expected for different genotypes. b Decreased mRNA levels of Rap1a without change in Rap1b in the cortex of Rap1a cKO mice (Left), decreased mRNA levels of Rap1b without change in Rap1a in the cortex of Rap1b cKO mice (Right). n = 3 mice for per genotype; Gapdh was used as loading controls. c Conditional deletion of either Rap1a or Rap1b in the forebrain had no effect on mouse body weight. n = 9 or 10 mice per group. Rap1af/f group, Rap1a cKO group and Rap1b cKO group (n = 14 mice); Rap1bf/f group (n = 15 mice). d Images of the whole brain at P56. e Rap1a or Rap1b knockout had no effect on brain weight. n = 9 or 10 mice per group. f Rap1a or Rap1b knockout had no effect on gross morphology of brain. Brain were subjected to Nissel staining at P56. Scale bar, 500 μm. Data are presented as mean ± SEM; *p < 0.05, **p < 0.01