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Fig. 4. | Cell & Bioscience

Fig. 4.

From: Disruption in iron homeostasis and impaired activity of iron-sulfur cluster containing proteins in the yeast model of Shwachman-Diamond syndrome

Fig. 4.

source for 40 hours in order to reach stationary phase following by extraction of proteins to determine the activity. Data shown are from three independent experiments. c Succinate dehydrogenase (SDH) activity was measured in WT, sdo1∆ and rho0 cells. The cells were cultured in YPD media with 2% glucose as a carbon source for 40 hours in order to reach stationary phase following by mitochondrial isolation and protein extraction for determining the SHD activity. Data shown are from three independent experiments. d and e Activities of aconitase and SDH were monitored in WT, sdo1∆ and sdo1∆ strain treated with BPS. BPS was added to the media 24 hours before culture. Cells were cultured and the activities of aconitase and SDH were analyzed as described in b and c respectively. Data shown are from three independent experiments. Values are the mean + SD. ***P < 0.001 and *P < 0.05, determined using Student’s T-test compared  to WT.

Cells lacking Sdo1p show a reduction in ISC enzyme activities. a The induction of FET3 utilizing FET3-lacZ reporter plasmid containing the lacZ gene under control of the FET3 element was monitored. WT and sdo1∆ cells were transformed with the plasmid pLJ440 (FET3-lacZ). Transformed cells were cultured in SC-URA selective media for 21 hours at 30°C until the OD600 of approximately 1.2. Data shown are from three independent experiments. b Aconitase activity was measured using whole cell lysates of WT, sdo1∆ and rho0 cells. The cells were cultured in YPD media with 2% glucose as a carbon

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