Fig. 3.

ROS production, protein oxidation, and H₂O₂ sensitivity are reduced in sdo1∆ yeast with iron depletion. a Intracellular ROS levels were monitored using DCFH-DA. The fluorescence intensity of each sample was normalized to protein content. Data shown are from three independent experiments. b Protein carbonyls were determined in whole cell lysates prepared from indicated strains untreated (H₂O₂: -) or treated with 2 mmol/L H₂O₂ (H₂O₂: +). DNPH-reactive carbonyl groups were detected by immunoblotting with an anti-DNP antibody. Immunostaining of phosphoglycerol kinase (Pgk1p) was used as loading control. c Quantitation of protein carbonylation results normalized to the intensity of Pgk1p with levels normalized to WT = 1. Data were from three independent experiments. d Growth of WT and sdo1∆ cells was monitored by spotting 10⁴, 10³, and 10² cells onto solid YPD medium alone (control) or supplemented with 120 µM BPS. The indicated concentrations of stressors were also included. Plates were incubated at 30 ˚C for 3 days and photographed. For heat stress, cells were incubated at 37 ˚C. Data are representative of three independent experiments. Values are the mean + SD. ***P < 0.001, **P <0.01, and *P < 0.05, determined using Student’s T-test compared between the means of two indicated groups.