ROS production, protein oxidation, and H2O2 sensitivity are reduced in sdo1∆ yeast with iron depletion. a Intracellular ROS levels were monitored using DCFH-DA. The fluorescence intensity of each sample was normalized to protein content. Data shown are from three independent experiments. b Protein carbonyls were determined in whole cell lysates prepared from indicated strains untreated (H2O2: -) or treated with 2 mmol/L H2O2 (H2O2: +). DNPH-reactive carbonyl groups were detected by immunoblotting with an anti-DNP antibody. Immunostaining of phosphoglycerol kinase (Pgk1p) was used as loading control. c Quantitation of protein carbonylation results normalized to the intensity of Pgk1p with levels normalized to WT = 1. Data were from three independent experiments. d Growth of WT and sdo1∆ cells was monitored by spotting 104, 103, and 102 cells onto solid YPD medium alone (control) or supplemented with 120 µM BPS. The indicated concentrations of stressors were also included. Plates were incubated at 30 ˚C for 3 days and photographed. For heat stress, cells were incubated at 37 ˚C. Data are representative of three independent experiments. Values are the mean + SD. ***P < 0.001, **P <0.01, and *P < 0.05, determined using Student’s T-test compared between the means of two indicated groups.