Over-accumulation of intracellular iron was observed in cells deficient for Sdo1p. a, b Wild type (WT), sdo1Δ, rho0, and ribosome defective mutants (dbp3∆, dbp7∆, dom34∆ and yar1∆) were cultured overnight in liquid YPD medium and the intracellular iron content was measured using graphite furnace atomic absorption spectrometry. Data shown are from three independent experiments. c Yeast were treated with 0.1 mg/mL cycloheximide (CHX) for 15 minutes to inhibit translation, intracellular iron levels were measured as described above. Data shown are from three independent experiments. Values are mean + SD, ***P <0.001, NS: not significant, determined using Student's T-test compared to WT.