Fig. 4

eMSC transduction and survival of eMSC on PA + G mesh in NSG mice. a cultured mCherry transduced eMSC showing red fluorescence, b more than 95% of transduced and cultured eMSC were mCherry + by flow cytometry and about 40% of this population were SUSD2+. Representative trace of n = 6 patient samples, c PA + G mesh seeded and cultured with eMSC. d, e mCherry + eMSC were observed 3 and f, g 7 days post-implantation around the mesh filaments in immunocompromised NSG mice. Arrows, representative mCherry + eMSC; m, mesh filament; g, gelatin. Scale Bars 100 µm. Reprinted with permissions from Darzi et al. [86]