Fig. 3

Observation of Osteogenesis and adipogenesis staining of ASCs and BMSCs. a Oil red staining for lipid droplets after 14-day lipogenic differentiation; BCIP/NBT staining for alkaline phosphatase after 7-day osteogenic differentiation; Alizarin red staining for mineralized nodules after 28-day osteogenic differentiation of ASCs and BMSCs. b Semi-quantitative comparison of BCIP/NBT staining using Image J software between ASCs and BMSCs. c Semi-quantitative comparison of Alizarin red staining using spectrophotometer at 620 nm between ASCs and BMSCs. a Oil red staining, scale bar = 50 μm; BCIP/NBT staining, scale bar = 200 μm; Alizarin red staining, scale bar = 200 μm. *P < 0.05; **P < 0.01