Fig. 5
The inhibitory role of silencing RPL34 on hilar cholangiocarcinoma cells. a QBC939 cells were infected with RPL34‑shRNA or NC lentivirus, and cell growth was assayed by the CCK-8 assay (shNT vs shRPL34). b QBC939 cells transfected with sh-RPL34 and the corresponding control cells (5*106) were suspended in 200 μl of PBS and then injected subcutaneously into the right flank of mice. Mice were sacrificed after 21 days, the tumors were dissected, and tumor volumes were measured. c Transwell migration assays were used to evaluate the effect of RPL34 on cell migration in QBC939 cells transfected with sh-RPL34. d The protein levels of E-cadherin, N-cadherin, and PCDH9 were measured by western blot in RPL34-silenced QBC939 cells. e The protein levels of PKM2, PCK2, and AKR1C1 were measured by western blot in RPL34-silenced QBC939 cells. **p < 0.01, *** p < 0.001, versus the shNC group. Magnification, × 100