Fig. 4

pERK is upregulated by Dzx treatment through ROS and is involved in upregulation of STIM1 and Orai1. a Upper panels, representative western blots of pERK from total extracts from isolated cardiomyocytes under the indicated experimental conditions. Middle panels, corresponding blots of total ERK. Lower panels, corresponding blots of GAPDH (loading control). The graph shows mean normalized pERK-band densities (± SEM, n = 4). Open circles represent single determinations. Arrows indicate the phosphorylated ERK (pERK, upper blot) and total ERK (ERK, middle blot) bands. b Representative western blots of pERK (upper blot) under the indicated experimental conditions. Middle panels, corresponding blots of total ERK. Lower panels, corresponding blots of GAPDH (loading control). The graph shows mean relative pERK expression levels (± SEM, n = 5). Open circles represent single determinations. c Representative western blots of STIM1 under the indicated experimental conditions. The graph shows mean STIM1 relative expression values (± SEM, n = 4). Open circles represent single determinations. d Representative western blots of Orai1 and actin under the indicated experimental conditions. The graph shows mean Orai1 relative expression values (± SEM, n = 4). Open circles represent single determinations. *p < 0.05, **p < 0.01