Fig. 3

Dzx effects on STIM1 and Orai1 expression and localization depend on synthesis de novo as revealed by the protein synthesis inhibitor CHX and mRNA determinations. a Representative western blots of STIM1 from cardiomyocytes incubated under the indicated experimental conditions. The graph shows mean normalized STIM1 band densities (± SEM, n = 4). Actin was used as a loading control. Open circles represent single determinations. b Upper panels, confocal microscopy images of cardiomyocytes under the experimental conditions indicated above the panels. Images show localization of STIM1 (red) with DAPI-labeled nuclei (blue). Lower panels are enlargements of the corresponding yellow boxed areas. c Representative western blots of Orai1 from cardiomyocytes incubated under the indicated experimental conditions. The graph shows mean normalized Orai1 band densities (± SEM, n = 4). Actin was used as a loading control. Open circles represent single determinations. d Confocal microscopy images of cardiomyocytes under the experimental conditions indicated above the panels. Images show localization of Orai1 (green) with DAPI-labeled nuclei (blue). Calibration bars, 10 μm in all cases. The graph shows mean numbers of particles (± SEM, n = 10) under the indicated experimental conditions. e, f Mean relative values (± SEM, n = 9–11) of mRNA expression from cardiomyocytes incubated in Dzx at the indicated times (filled bars). **p < 0.01, *p < 0.05