Fig. 2

Upregulation and distribution changes of STIM1 and Orai1 proteins by Dzx depend on ROS and the opening of mKATP channels. a Representative western blots of STIM1 from whole membrane fractions of ventricles. The graph shows mean (± SEM, n = 5–15) normalized densities of STIM1 bands under the indicated experimental conditions. Open circles represent single determinations. b Representative western blots of Orai1 from whole membrane fractions of ventricles under experimental conditions as in a. The graph shows mean (± SEM, n = 4–23) normalized densities of Orai1 bands under the indicated experimental conditions. Actin was used as a loading control in all cases. Open circles represent single determinations. c Upper panels, representative confocal images of cardiomyocytes under the indicated experimental conditions. Lower panels, enlargements of yellow boxed areas. Images show localization of STIM1 (red) with DAPI-labeled nuclei (blue). d Representative confocal images of cardiomyocytes under the indicated experimental conditions. Images show localization of Orai1 (green) with DAPI-labeled nuclei (blue). Calibration bars, 10 μm in all cases. e Plot fluorescence intensity profiles along the longitudinal axis of the cardiomyocytes shown in c. f Particle analysis of cardiomyocytes under the same conditions as in d. Mean particle counts (± SEM, n = 5–7) are shown. *p < 0.05, **p < 0.01