Table 1 Primers, amplicon size and annealing temperature of ZnT3, Sp1 and β-actin for RT-PCR and ChIP-PCR
Name | Primer sequences | Amplicon size (bp) | Annealing temperature | |
|---|---|---|---|---|
Forward | Reverse | |||
β-actin | GTCGTACCACAGGCATTGTGATGG | GCAATGCCTGGGTACATGGTGG | 492 | 58/63 °C |
β-actin | TTTCCAGCCTTCCTTCTTGGGTATG | ATAGAGGTCTTTACGGATGTCAACG | 100 | 58/63 °C |
ZnT3 | GGAGGTGGTTGGTGGGTATTTAGC | CCATTAGCAGATTGGCACAGACAGC | 317 | 58 °C |
Sp1 | AGAACCCACAAGCCCAGACAATC | CTCCTTCTCCACCTGCTGTCTCA | 330 | 63 °C |
ZnT3 promoter -338 ~ -232(GC-1) | GTCGTACCACAGGCATTGTGATGG | GCAATGCCTGGGTACATGGTGG | 106 | 60 °C |
ZnT3 promoter -253 ~ -112(GC-2) | TTTCCAGCCTTCCTTCTTGGGTATG | ATAGAGGTCTTTACGGATGTCAACG | 141 | 60 °C |