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Fig. 5 | Cell & Bioscience

Fig. 5

From: Disruption of zinc transporter ZnT3 transcriptional activity and synaptic vesicular zinc in the brain of Huntington’s disease transgenic mouse

Fig. 5

Sp1 up-regulates ZnT3 gene transcriptional activity. a The scheme chart of the mouse ZnT3 promoter sequence. Potential Sp1 binding sites (GC-1 and GC-2) are underlined. b Dual-luciferase (Luc) report gene assay of the targeting relationship of Sp1 and ZnT3. Sp1 activates ZnT3 transcription by binding to GC boxes in ZnT3 promoter. n = 3 *p < 0.05 compared to BHK cells transfected with empty pEBGN vector. (C) ChIP analysis of Sp1 binding to ZnT3 promoter. The relative amounts of ZnT3 promoter were normalized to input DNA. GC-1: ZnT3 promoter (−338 to  −232); GC-2:ZnT3 promoter (−253 to  −112). n = 3. *p < 0.05 compared to control IgG. d–g The effect of Sp1 on ZnT3 expression. ZnT3 expression level greatly increase in BHK cells overexpressing Sp1 (d Western blot analysis, e RT-PCR analysis). In contrast, ZnT3 expression level decreases in BHK cells transfected with Sp1 siRNA (f Western blot analysis, g RT-PCR analysis). n = 4. *p < 0.05 compared to BHK cells transfected with empty vector

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