Fig. 4

CD44 and αvβ3 were upregulated in SCs after sciatic nerve injury in rats. a Real-time qPCR detected the expression of integrin subunits αv, α1, α2, α3, α4, α5, α6, α7, α8, α9, and β1, β3, β4, β5, β6, β8 in SCs. b and c Immunofluorescence staining of CD44 (red), αv (red), and S100β (green) in SCs. b CD44 mainly located at the cytomembrane. c αv mainly concentrated at the focal adhesion. Scale bar, 50 and 25 μm. d Immunofluorescence staining of CD44 (green) and S100β (red) in the distal sciatic nerve stumps at 0, 1, 4, 7, 14, and 28 days after injury. Scale bar, 200 and 50 μm. e Immunofluorescence staining of αv (red) and S100β (green) in the distal sciatic nerve stumps at 0, 1, 4, 7, 14, and 28 days after injury. Scale bar, 200 and 50 μm. f–i Western blot analysis of CD44, αv, and β3 in injured sciatic nerve at 0, 1, 4, 7, 14, and 28 days after injury. f Representative blot. g–i Quantification of relative expression of proteins in (f). j–l Real-time qPCR analyzed the relative mRNA expression of CD44, αv, and β3 in injured sciatic nerve at 0, 1, 4, 7, 14, and 28 days. Data shown as mean ± SEM from triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001