Fig. 1From: SPP1 promotes Schwann cell proliferation and survival through PKCα by binding with CD44 and αvβ3 after peripheral nerve injuryExpression of SPP1 and PKCα was increased in SCs after median nerve injury in the human specimens. a Immunofluorescence staining of SPP1 (red), PKCα (red) and S100β (green) in health median nerve and distal median nerve stumps at 5 days after injury. Scale bar, 200 and 50 μm. b, c Relative mRNA expression of SPP1 (b) and PKCα (c) in injured median nerve at 0, 5, 8, 13 h, 7, and 14 days of six independent samples by real-time qPCR. d–f Western blot analysis of SPP1 and PKCα in injured median nerve at 0, 5, 8, 13 h, 7, and 14 days after injury. Repeated for three-time. d Representative blot results. e, f Quantification of the relative expression of proteins in (d). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001Back to article page