Fig. 6

Nrf2 enhances radiation resistance by up-regulating the expression of CaMKIIα in ESCC cells. The expression levels of phosphorylated CaMKIIα and CaMKIIα in wild-type (WT) and Nrf2 overexpressed (Nrf2-OE) ESCC cells were detected by western blotting (a for Ec109 cells, b for KYSE-30 cells). Nrf2-OE Ec109 cells were pretreated with 15 μM KN-93 for 12 h and then irradiated with 4 Gy X-ray. After that, the radiation resistance was detected by Cell counting kit-8 (CCK-8) (c) and colony formation test (d). All results are presented as the mean ± SEM from three repeated experiments. The differences among the groups were obtained using Analysis of Variance (ANOVA). *P < 0.05, **P < 0.01, and ***P < 0.001