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Table 1 List of primers used for quantitative polymerase chain reaction (β-actin was used as an internal control)

From: Macrophages inhibit adipogenic differentiation of adipose tissue derived mesenchymal stem/stromal cells by producing pro-inflammatory cytokines

Gene Primer sequences
PPAR-γ
 Forward 5′-TACTGTCGGTTTCAGAAATGCC-3′
 Reverse 5′-GTCAGCGGACTCTGGATTCAG-3′
C/EBP-α
 Forward 5′-CGAAGAGACGGCCCTTGCTG-3′
 Reverse 5′-GGGATACATCCTCAGGGCCACA-3′
C/EBP-β
 Forward 5′-CTTCAGCCCGTACCTGGAG-3′
 Reverse 5′-GGAGAGGAAGTCGTGGTGC-3′
C/EBP-γ
 Forward 5′-ACTCCAGGGGTGAACGGAAT-3′
 Reverse 5′-CATGGGCGAACTCTTTTTGCT-3′
Glut4  
 Forward 5′-TGGGCGGCATGATTTCCTC-3′
 Reverse 5′-GCCAGGACATTGTTGACCAG-3′
LPL
 Forward 5′-TCATTCCCGGAGTAGCAGAGT-3′
 Reverse 5′-GGCCACAAGTTTTGGCACC-3′
FABP4
 Forward 5′-ACTGGGCCAGGAATTTGACG-3′
 Reverse 5′-CTCGTGGAAGTGACGCCTT-3′
IL-6
 Forward 5′-CAGCCCTGAGAAAGGAGACATG-3′
 Reverse 5′-GGTTGTTTTCTGCCAGTGCCT-3′
IL-12
 Forward 5′-GATGGCCCTGTGCCTTAGTA-3′
 Reverse 5′-TCAAGGGAGGATTTTTGTGG-3′
IL-1β  
 Forward 5′-GACCTGAGCACCTTCTTTCCCT-3′
 Reverse 5′-CATCGTGCACATAAGCCTCGT-3′
TNF-α
 Forward 5′-GACAAGCCTGTAGCCCATGTTG-3′
 Reverse 5′-TGGTTATCTCTCAGCTCCACGC-3′
TGM2
 Forward 5′-CAAGGCCCGTTTTCCACTAAG-3′
 Reverse 5′-GAGGCGATACAGGCCGATG-3′
CCL22
 Forward 5′-ATCGCCTACAGACTGCACTC-3′
 Reverse 5′-GACGGTAACGGACGTAATCAC-3′
β-actin
 Forward 5′-TTGCCGACAGGATGCAGAAGGA-3′
 Reverse 5′-AGGTGGACAGCGAGGCCAGGAT-3′
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Cell & Bioscience

ISSN: 2045-3701

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