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Fig. 1 | Cell & Bioscience

Fig. 1

From: Macrophages inhibit adipogenic differentiation of adipose tissue derived mesenchymal stem/stromal cells by producing pro-inflammatory cytokines

Fig. 1

Macrophage-derived supernatants inhibit adipocyte differentiation of hADSCs. a Scheme of the experimental procedure. hADSCs (2 × 104) were plated and cultured with adipogenesis-inducing medium (AD) with or without the supernatants from different macrophage subtypes at the ratio of 1:1 in 48-well plate, medium was changed every 3 days. b hADSCs cells that were induced to undergo adipogenic differentiation were fixed for triglycerides staining with Oil Red O to show lipid droplets. c Western blot analysis of the protein levels of PPAR-γ in hADSCs cultured in different conditions for 5 days. d Expression of adipogenic genes were measured by Q-PCR assay on day 3. e hADSCs were pre-treated with the specific supernatant for 24 h, then changed to adipogenic inducing medium without supernatant, lipid droplets in hADSCs were revealed by Oil Red O staining after 9 days. Scale bars are 100 μm

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