Table 1 Primer sequences used in real-time PCR analysis
cDNA | Primer sequences | Product length (bp) |
|---|---|---|
Mus-Foxp3 (NM_054039) | F:5′-CAGCTCTGCTGGCGAAAGTG-3′ R:5′-TCGTCTGAAGGCAGAGTCAGGA-3′ | 123 |
Mus-Il-10 (NM-010548) | F:5′-GGGGCCAGTACAGCCGGGAA-3′ R:5′-CTGGCTGAAGGCAGTCCGCA-3′ | 92 |
Mus-Ifn-γ (NM-008337) | F:5′-AAGCGTCATTGAATCACACCTG-3′ R:5′-TGACCTCAAACTTGGCAATACTC-3′ | 92 |
Mus-Il-1β (NM_008361) | F:5′-TGCCACCTTTTGACAGTGATG-3′ R:5′-AAGGTCCACGGGAAAGACAC-3′ | 220 |
Mus-Tnf-α (NM_013693) | F:5′-CCTGTAGCCCACGTCGTAG-3′ R:5′-GGGAGTAGACAAGGTACAACCC-3′ | 148 |
Mus-Il-12 (NM_001303244) | F:5′-TGGTTTGCCATCGTTTTGCTG-3′ R:5′-ACAGGTGAGGTTCACTGTTTCT-3′ | 123 |
Mus-Il-6 (NM_031168) | F:5′-CTGCAAGAGACTTCCATCCAG-3′ R:5′-AGTGGTATAGACAGGTCTGTTGG-3′ | 131 |
Mus-Gapdh (NM_008084) | F:5′-TGTGTCCGTCGTGGATCTGA-3′ R:5′-TTGCTGTTGAAGTCGCAGGAG-3′ | 150 |