Fig. 4
Chronic arsenic exposure up-regulates nuclear factor NRF2 and its downstream targets in spleen. Mice were treated with NaAsO2 (25 mg/L and 50 mg/L) for 1, 3 and 12 months through drinking water ad libitum. Splenic NRF, GSTO1/2 and GCLC protein levels by treatment of a 25 mg/L NaAsO2 for 1, 3 and 12 months and b 25 mg/L and 50 mg/L NaAsO2 for 12 months were assessed by western blot. β-actin was blotted as the internal control. The exposure duration of the control group was 12 months in a and b. The density quantification of each band was presented as mean ± SD (n = 3)