Fig. 3
From: Genome editing of CCR5 by AsCpf1 renders CD4+T cells resistance to HIV-1 infection
The CRISPR/AsCpf1-#4/#5 expressing lentivirus induced genome editing of CCR5 in SupT1-R5 cells. a 300 ng PCR products of #4 and #5 or control were analyzed by T7E1 assay after lenti-CRISPR/AsCpf1 mediated CCR5 disruption at day 3. b The amplicons of #4 and #5 were treated with poly A adding kit, and the products were further ligated with PGEM-T easy vector and analyzed with DNA sequencing. c CCR5 protein level of the edited SupT1-R5 cells were detected at day 7 with 10 µg load after packaged lentivirus transduction. d The CCR5 expression on cell surface were assessed with FACS