Fig. 2
From: Genome editing of CCR5 by AsCpf1 renders CD4+T cells resistance to HIV-1 infection
Lenti-CRISPR/AsCpf1 mediated the disruption of CCR5 in TZM.bl cells. a 5 sgRNAs for CCR5 editing by CRISPR/AsCpf1 were screened and identified by T7 endonuclease 1 (T7E1). b The amplicons of #4 and #5 were subjected to DNA sequencing by ligating with PGEM-T easy vector. c the protein level of CCR5 on cell surface were analyzed by flow cytometry at the third day post-transfection